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First single locus triple R gene stack potatoes

For the first time, using a single vector and one-step transformation method, researchers have produced genetically modified potatoes carrying three broad-spectrum resistance alleles to the fungal pathogen, late blight ( Phytophthora infestans - Pi). The researchers combined resistance genes (Rpi) from three potato species, Rpi-sto1 from Solanum stoloniferum, Rpi-vnt1.1 ( S venturii) and Rpi-blb3 ( S bulbocastanum) into a single vector that was then transformed into a susceptible potato cultivar, Desiree. Results from detached leaf assays showed that transformants challenged with the three corresponding avirulence (Avr) effectors mounted a hypersensitive response (HR), indicating the successful integration of all three R genes.


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