Quick multiplex assay IDs four pathogens
Researchers from the UK’s Queens University Belfast and Thailand’s National Science and Technology Development Agency (Klong Luang) have developed the first multiplex microsphere immunoassay for the simultaneous detection of four plant pathogens. The new test, for fruit blotch ( Acidovorax avenae subsp citrulli), chilli vein-banding mottle virus, watermelon silver mottle virus and melon yellow spot virus has “substantially higher sensitivity” than enzyme-linked immunosorbent assays (ELISA), and is much faster, with results available in one hour as opposed to four hours from ELISA, the researchers say. The system can detect the pathogens in naturally infected plant samples and is a major advancement in plant pathogen detection, they note. Details of the method have been published in the journal, PLoS One.